The essential oils of Chamaecyparis obtusa promote hair growth in an animal model. - GreenMedInfo Summary
The essential oils of Chamaecyparis obtusa promote hair growth through the induction of vascular endothelial growth factor gene.
Fitoterapia. 2009 Jul 1. PMID: 19576968
Chamaecyparis obtusa (C. obtusa) is a conifer in the cypress family Cupressaceae, native to northeast Asia. The essential oils of C. obtusa have antibacterial and antifungal effects and several products such as hygienic bands, aromatics, and shampoos contain these oils as a natural source of antimicrobial/antifungal agents. Interestingly, some consumers suffering from baldness and/or other forms of hair loss have reported a hair growth promoting effect of shampoos containing these oils. In the present study, the hair growth promoting effect of C. obtusa oils was elucidated in an animal model. C. obtusa oils promoted the early phase of hair growth in shaved mice. In addition, we examined the molecular effect of C. obtuse oils on the regulation of hair morphogenesis and hair growth using the human keratinocyte cell line HaCaT. In current study of hair growth regulating genes, the expressions of vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGFbeta1), and keratinocyte growth factor (KGF) have been analyzed by real-time PCR in HaCaT cells. The essential oils of C. obtusa were divided into seven fractions for treatment of HaCaT cells. VEGF transcripts were induced by fraction 6 and 7; however, TGFbeta1 and KGF mRNAs levels were unchanged by C. obtusa oils or fractions. Fraction 7 was separated into seven sub-fractions and studied further. Sub-fractions E and D significantly increased VEGF and KGF gene expression without up-regulating the hair growth inhibition factor, TGFbeta. The components of the two sub-fractions were further analyzed by gas chromatography and mass spectrometry. Cuminol, eucarvone, and calamenene were common to these two sub-fractions, although the effects of these individual components were not determined. Taken together, these results suggest that C. obtusa oils promote hair growth in an animal model and a positive regulator of hair growth, VEGF, was induced by particular components of these oils.