Ethanolic Extracts fromLeaves Modulate Transcriptional Levels of Hormone Receptor Variant in Breast Cancer Cell Lines.
Int J Mol Sci. 2018 Jun 26 ;19(7). Epub 2018 Jun 26. PMID: 29949923
Deisi L Braga
Breast Cancer (BC) encompasses numerous entities with different biological and behavioral characteristics, favored by tumor molecular complexity.(neem) presents phenolic compounds, indicating its potential as an antineoplastic compound. The present study aimed to evaluate the cellular response of MCF10, MCF7, and MDA-MB-231 breast cell lines to ethanolic extracts of neem leaves (EENL) obtained by dichloromethane (DCM) and ethyl acetate (EA) solvent. Extracts’ antiproliferative activities were evaluated against MCF 10A, MCF7, and MDA-MB-231 for 24 and 48 h using MTT assay.,,,,andtranscripts were quantified through qPCR for 0.03125μg/mL of DCM and 1.0μg/mL for EA for 48 h. The EENL was tested onas a sole treatment and then also together with doxorubicin. Antiproliferative effect on tumor cell lines without affecting MCF 10A were 1.0µg/mL (<0.001) for EA, and 0.03125µg/mL (<0.0001) for DCM, both after 48 h. Transcriptional levels ofincreased after treatment. In vivo assays demonstrated that EENL induced fewer tumors at a higher concentration with doxorubicin (DXR). The behavior ofin the MDA-MB-231 tumor lineage indicates new pathways involved in tumor biology and this may have therapeutic value for cancer.