Cytotoxic analysis and chemical characterization of fractions of the hydroalcoholic extract of the Euterpe oleracea Mart. seed in the MCF-7 cell line.
J Pharm Pharmacol. 2017 Jun ;69(6):714-721. Epub 2017 Feb 17. PMID: 28211563
Dayanne da S Freitas
OBJECTIVES: To analyse the antineoplastic activity of fractions derived from the hydroalcoholic extract of Euterpe oleracea Mart. seed in the MCF-7 cell line and to identify the compounds responsible for the antineoplastic action.
METHODS: Cells were treated with 10, 20, 40 and 60μg/ml with the hexane, chloroform and ethyl acetate fraction (EAF) of the hydroalcoholic extract of açaí seed, for 24 and 48 h. After treatment, cell viability was measured using MTT assay and cell death was assessed using the Annexin-Pi assay. The most cytotoxic fraction under study was analysedby mass spectrometry using an electrospray ionization source and a cyclotron analyser coupled to a Fourier transform. Data were analysed statistically by analysis of variance (ANOVA) or by Student's t-test, where appropriate.
KEY FINDINGS: All fractions caused significant reduction in the cell viability, but the EAF was the most cytotoxic (P<0.001). It was observed the absence of significant annexin staining but increase Pi staining (P<0.001). The EAF is composed of epicatechin, proanthocyanidin A2 and trimeric and tetrameric procyanidins.
CONCLUSIONS: In this study, we demonstrated that EAF was the most effective fraction in reducing cell viability and causing necroptosis in the MCF-7 cell.