Evaluation of the Antibacterial Activity and Efflux Pump Reversal of Thymol and Carvacrol againstand Their Toxicity in.
Molecules. 2020 Apr 30 ;25(9). Epub 2020 Apr 30. PMID: 32365898
Zildene de Sousa Silveira
The antibacterial activity and efflux pump reversal of thymol and carvacrol were investigated against theIS-58 strain in this study, as well as their toxicity against. The minimum inhibitory concentration (MIC) was determined using the broth microdilution method, while efflux pump inhibition was assessed by reduction of the antibiotic and ethidium bromide (EtBr) MICs.toxicity was tested using the fumigation method. Both thymol and carvacrol presented antibacterial activities with MICs of 72 and 256µg/mL, respectively. The association between thymol and tetracycline demonstrated synergism, while the association between carvacrol and tetracycline presented antagonism. The compound and EtBr combinations did not differ from controls. Thymol and carvacrol toxicity againstwere evidenced with ECvalues of 17.96 and 16.97µg/mL, respectively, with 48 h of exposure. In conclusion, the compounds presented promising antibacterial activity against the tested strain, although no efficacy was observed in terms of efflux pump inhibition.