Evaluation of the Anticancer Activity of Sprout Extract-Loaded Nanoemulsion of N. sativa against Hepatocellular Carcinoma.
J Microencapsul. 2019 Jan 23:1-42. Epub 2019 Jan 23. PMID: 30669915
Nigella sativa L. belonging to Ranunculaceae family is an important medicinal spice which has been utilized to treat various chronic diseases. Lipid nanoemulsions containing oil from medicinal plants have shown to enhance drug dissolvability, diminish symptoms of different powerful medications and enhance the bioavailability of medications, in contrast with conventional formulations. In the present study, aqueous titration method was used to prepare nanoemulsion. The optimized formulation (NE) with the mean particle size of 37.47 nm showed a minimum viscosity of 0.547cps and maximum drug release (98.2%) in 24 h. The stability study showed considerably stable formulations at refrigerator temperature as compared to room temperature. The cancer cell line studies confirmed that 5d sprout extract of N. sativa nanoemulsion reduced the cell viability (p<0.05) and increased colony formation, ROS intensity and chromatin condensation. All data such as colony formation, ROS intensity and chromatin condensation are represented as mean ± SD (p<0.001) treated cells for 48hours. Our results concluded that the development of nanoemulsion could be an efficient carrier for the drug delivery.