Expression profiles of long noncoding RNAs in mice with high-altitude hypoxia-induced brain injury treated with Gymnadenia conopsea. - GreenMedInfo Summary
Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with(L.) R. Br.
Neuropsychiatr Dis Treat. 2020 ;16:1239-1248. Epub 2020 May 12. PMID: 32494143
Yongcang Zhang
Background: The unique geographical environment at high altitudes may cause a series of diseases, such as acute altitude reaction, cerebral edema, and pulmonary edema.(L.) R. Br. has been reported to have an effect on high-altitude hypoxia. However, the molecular mechanism, especially the expression of long noncoding RNAs (lncRNAs), is not yet clear.
Methods: The expression profiles of lncRNAs in high-altitude hypoxia-induced brain injury mice treated with(L.) R. Br. by using a microarray method.
Results: A total of 226 differentially expressed lncRNAs, 126 significantly dysregulated mRNAs and 23 differentially expressed circRNAs were detected (>2.0-fold, p<0.05). The expression of selected lncRNAs, mRNAs and circRNAs was validated by qRT-PCR. KEGG analysis showed that the mRNAs coexpressed with lncRNAs were involved in inflammation and hypoxia pathways, including the HIF-1, PI3K-Akt, and NF-kappa B signaling pathways. The lncRNA-TF network analysis results indicated that the lncRNAs were regulated mostly by HMGA2, SRY, GATA4, SOX5, and ZBTB16.
Conclusion: This study is the first to report the expression profiles of lncRNAs, mRNAs and circRNAs in mice with high-altitude hypoxia-induced brain injury treated with(L.) R. Br. and may improve the understanding of the molecular mechanism of(L.) R. Br. in treating high altitude hypoxia-induced brain injury.