Extracts and components ofleaves suppress survival, cell cycle, and migration of triple-negative breast cancer MDA-MB-231 cells.
Onco Targets Ther. 2018 ;11:4377-4386. Epub 2018 Jul 27. PMID: 30100743
Background: Products fromhave been used in traditional medicine to treat many diseases. This study aimed to analyze anticancer effects of extracts ofleaves on the triple-negative breast cancer cell line MDA-MB-231.
Materials and methods: The human breast cancer cell line MDA-MB-231 was used to evaluate effects ofextracts. Effects ofon cell viability were evaluated using MTT assays. Cell-cycle distribution was examined using cell-cycle analysis. Wound-healing assays were used to evaluate migration of MDA-MB-231. Quantitative reverse-transcription polymerase chain reaction was used to detect levels of,,,,, cyclin-dependent kinases,, and tissue inhibitors of metalloproteinase.
Results: We investigated the mechanism of anti-growth effects, and found that the expressions of genes that promote apoptosis were increased. In addition, the treated cells illustrated increased portion at S phase and changed expression of cyclin-dependent kinases, demonstrating cell-cycle arrest at the S phase. Furthermore, treated cells showed decreased cell mobility, which is essential for metastasis. Two of the active components ofleaves, bergapten and psoralen, had similar anticancer effects asleaf extracts, indicating that these two components might play important roles in anticancer effects ofleaves.
Conclusion: Our findings suggest thatleaves might be a good source to develop drugs for suppressing cancer-cell growth and migration to treat triple-negative breast cancers.