Article Publish Status: FREE
Abstract Title:

Apoptosis-inducing effects of jujube (Zǎo) seed extracts on human Jurkat leukemia T cells.

Abstract Source:

Chin Med. 2016 ;11:15. Epub 2016 Apr 1. PMID: 27042202

Abstract Author(s):

Natthanan Taechakulwanijya, Natthida Weerapreeyakul, Sahapat Barusrux, Sirithorn Siriamornpun

Article Affiliation:

Natthanan Taechakulwanijya


BACKGROUND: Jujube (Zǎo) seeds exhibited anticancer effects and used in Chinese medicine for many years. This study aims to investigate the apoptosis-inducing effects of seed extracts from eight different cultivated species ('Apple', 'Bombay', 'Jumbo', 'Kaew', 'Nomsod', 'Rianthong', 'Samros', and 'Taiwan') on human Jurkat leukemia T cells.

METHODS: We evaluated the effects of seed extracts from eight jujube cultivated species on human Jurkat leukemia T cells. The crude seed extracts were prepared sequentially by using water, 95 % ethanol, dichloromethane, ethyl acetate, chloroform or hexane. The antiproliferative effects of the jujube seed extracts relative to that of melphalan were evaluated by neutral red assays. Apoptotic cell death induced by the ethanolic extracts at 1 × IC50 and 2 × IC50 concentrations was demonstrated by DAPI staining, gel electrophoresis, flow cytometry with Annexin V/propidium iodide staining, and caspase-3, -8, and -9 enzyme activities.

RESULTS: Ethanolic extracts of 'Taiwan', 'Jumbo', 'Nomsod', 'Rianthong', 'Samros', and 'Bombay', significantly inhibited the proliferation of Jurkat cells compared with untreated cells (all P < 0.001), while the extracts of 'Kaew' and 'Apple' were inactive. The six active extracts preferentially induced apoptotic cell death in a concentration-dependent manner with DNA fragmentation (2 × IC50). Increased caspase-3 activity was detected after treatment with the six extracts. The 'Taiwan', 'Nomsod', 'Jumbo', and 'Rianthong' extracts (2 × IC50) induced both the extrinsic and intrinsic apoptosis pathways by increasing caspase-8 and caspase-9 activity, respectively. Alkaloids (Dragendorff's method) and reducing sugars (Fehling's test) were mainly identified in the apoptosis-inducing extracts.

CONCLUSIONS: The tested of six active extracts ('Taiwan', 'Jumbo', 'Nomsod', 'Rianthong', 'Samros' and 'Bombay') contained alkaloids or reducing sugars, and induced caspase-dependent apoptosis in human Jurkat leukemia T cells.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021, Journal Articles copyright of original owners, MeSH copyright NLM.