Modulatory Effects of Rosemary Leaves Aqueous Extraction against oxidative stress and related damages in experimental model of CCl4-induced cardiac toxicity in mice.
Appl Physiol Nutr Metab. 2018 Apr 9. Epub 2018 Apr 9. PMID: 29629570
Rosmarinus officialis L., Lamiaceae, has been traditionally used in Tunisia to treat several diseases. The aim of the present study is to analyze the cardiopreventive effect of Aqueous Extract of Rosemary (Rosmarinus officinalis) Leaves (AERL) against oxidative stress induced by carbon tetrachloride in experimental mice. The current study was performed on female Wistar mice divided into four groups of seven animals each. Cardiotoxity was induced by a single intraperitoneal injection of CCl4 (10ml/kg in 0.3 % olive oil) in mice which were gavaged daily with 100 mg of AERL for 15 days. CCl4 exposure caused a disturbance of cardiac markers such as LDH,γGT, CK, CKMB and Troponin I. Moreover, cardiotoxicity indices such as TBARS and protein carbonyls levels exhibited remarkable increases, while antioxidant enzymes activities (CAT, SOD and GPx) and GSH level decreased when compared with control group. Besides, the triglycerides, the total cholesterol, and low-density lipoprotein cholesterol levels and the transaminases activities (ALT, AST and ALP) decreased. The pretreatment with AERL can also significantly prevent the heart damage through the correction of electrolytes (Na+, K+, Cl- and Ca2+) and lipid parameters, leading to a good defenseagainst cardiotoxicity as well as good antioxidant status. The histological analysis of cardiac tissues further established the positive effect of AERL. These findings could be due to the high phenolic content and to important antioxidant capacity of Rosmarinus officinalis. Overall, this study suggests that the administration of AERL displays high potential to quench free radicals and alleviates CCl4-induced cardiotoxicity in mice.