The Leaf Extracts ofand Fermented Culture Broths ofSynergistically Cause Apoptotic Cell Death in Promyelocytic Leukemia Cells.
Integr Cancer Ther. 2020 Jan-Dec;19:1534735420923734. PMID: 32618215
is a common edible vegetable that is used in certain Chinese dishes and has importance in folk medicine. The leaf extracts ofpossess and exhibit anticancer efficacy against various cancer cell types. In Taiwanese folklore,, also known as"Niu-Cheng-Zi,"is used in traditional medicine to treat various illnesses. Its fruit and mycelium possess various potent antiproliferative properties. Two studies from our group have reported thatorhas the ability to cause apoptosis in various cancer cells. Conversely, underlying molecular mechanisms and any beneficial effects remain unknown. This study shows anticancer efficacy for bothandco-treatments that target HL-60 cells. The combination index values indicate that 40µg/mL ofand 25µg/mL ofas a combined treatment shows a synergetic effect, which reduces HL-60 cell proliferation. Alternately, this treatment exhibited no cytotoxic effects for human umbilical vein endothelial cells. Western blot data showed thatandas a combined treatment result in augmented expression of apoptosis, cytochrome c release, Bcl-2 inhibition, expression of Bax, Fas, and FasL, as well as the cleavage of Bid in HL-60 cells. Moreover, this combined treatment overshadowed monotherapy in its ability to inhibit uPAR, MMP-9, MMP-2, COX-2 expression, and PGEsecretions. Our study strongly implies that this combined treatment offers more beneficial effects to suppress and treat leukemia due to apoptosis-mediated cell inhibition. Furtherstudies related to the combined treatment could establish its future potential.