"Female But Not Male Mice Show Delayed Cutaneous Wound Healing By Aspirin Administration." - GreenMedInfo Summary
Female But Not Male Mice Show Delayed Cutaneous Wound Healing By Aspirin Administration.
Clin Exp Pharmacol Physiol. 2012 Dec 14. Epub 2012 Dec 14. PMID: 23240590
Department of Histology and Embryology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Cyclooxygenase (COX) is an enzyme that participates in the wound healing process. Aspirin, a nonsteroidal anti-inflammatory drug, simultaneously inhibits COX-1 and -2 isoforms' aromatase activity, which are needed for prostaglandins synthesis. The aim of this study was to evaluate whether aspirin, and thus COX inhibition, distinctly affects cutaneous wound healing in female and male mice. Female and male Balb/c mice were treated with aspirin (25 mg/kg/day) until euthanasia. The control group received vehicle only. A full-thickness excisional lesion was performed, and macroscopic, histological and biochemical parameters were evaluated. Sections were stained and immunostained for microscopic analysis. Myeloperoxidase activity, hydroxyproline quantity and protein expression of von Willebrand factor (vWF) and vascular endothelial growth factor (VEGF) were also measured. Female control and treated groups presented delayed wound closure and reepithelization when compared to male control and treated groups, respectively. The female control group presented reduced myeloperoxidase activity and MIF-positive cells compared to the male control group. In the female treated group, myeloperoxidase activity and F4/80-positive macrophage number was higher than the control group. Collagen was reduced only in the female treated group. vWF and VEGF protein expression were increased in the female-treated group. In summary, aspirin administration impaired the wound healing process in Balb/c females but not in males.© 2012 The Authors Clinical and Experimental Pharmacology and Physiology © 2012 Wiley Publishing Asia Pty Ltd.