Isolation and characterization of a French bean hemagglutinin with antitumor, antifungal, and anti-HIV-1 reverse transcriptase activities and an exceptionally high yield.
Phytomedicine. 2009 Sep 7. Epub 2009 Sep 7. PMID: 19740639
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
A dimeric 64-kDa hemagglutinin was isolated with a high yield from dried Phaseolus vulgaris cultivar "French bean number 35" seeds using a chromatographic protocol that involved Blue-Sepharose, Q-Sepharose, and Superdex 75. The yield was exceptionally high (1.1g hemagglutinin per 100g seed), which is around 10-85 times higher than other Phaseolus cultivars. Its N-terminal sequence resembled those of other Phaseolus hemagglutinins. The hemagglutinating activity of the hemagglutinin was stable in the pH range 6-8, and in the temperature range 0 degrees C-50 degrees C. It inhibited HIV-1 reverse transcriptase with an IC(50) of 2muM. It suppressed mycelial growth in Valsa mali with an IC(50) of 10muM. It inhibited proliferation of hepatoma HepG2 cells and breast cancer MCF-7 cells with an IC(50) of 100 and 2muM, respectively. It had no antiproliferative effect on normal embryonic liver WRL68 cells.