In vitro and in silico inhibition properties of fucoidan againstα-amylase and α-D-glucosidase with relevance to type 2 diabetes mellitus.
Carbohydr Polym. 2019 Apr 1 ;209:350-355. Epub 2019 Jan 14. PMID: 30732817
Lakshmana Senthil S
This study investigated the in vitro and in silico inhibitory properties of fucoidan extracted from Turbinaria conoides againstα-amylase and α-D-glucosidase. Extracted fucoidan contained 59 and 35% of fucose and sulphate and was characterized usingH NMR. Dose dependent inhibition assays showed maximum of 85 and 72% of inhibition forα-amylase and α-D-glucosidase at 2.07 and 1.03 μM concentration of fucoidan. The ICvalue of fucoidan was found to be 1.07 and 0.68 μM against α-amylase and α-D-glucosidase. In silico studies (grid based docking) by Schrödinger software revealed that fucoidan as a potent inhibitor for both α-amylase and α-D-glucosidase based on number of interactions, hydrogen bond length and binding energy. Furthermore, fucoidan fulfilled the pharmacokinetic properties thus promising to develop fucoidan as drug for type 2 DM.