Gallic acid inhibits aflatoxin formation in Aspergillus flavus. - GreenMedInfo Summary
The Antioxidant Gallic Acid Inhibits Aflatoxin Formation inby Modulating Transcription Factors FarB and CreA.
Toxins (Basel). 2018 Jul 3 ;10(7). Epub 2018 Jul 3. PMID: 29970790
Aflatoxin biosynthesis is correlated with oxidative stress and is proposed to function as a secondary defense mechanism to redundant intracellular reactive oxygen species (ROS). We find that the antioxidant gallic acid inhibits aflatoxin formation and growth inin a dose-dependent manner. Global expression analysis (RNA-Seq) of gallic acid-treatedshowed that 0.8% (/) gallic acid revealed two possible routes of aflatoxin inhibition. Gallic acid significantly inhibited the expression of, encoding a transcription factor that participates in peroxisomal fatty acidβ-oxidation, a fundamental contributor to aflatoxin production. Secondly, the carbon repression regulator encoding gene,, was significantly down regulated by gallic acid treatment. CreA is necessary for aflatoxin synthesis, and aflatoxin biosynthesis genes were significantly downregulated in∆mutants. In addition, the results of antioxidant enzyme activities and the lipid oxidation levels coupled with RNA-Seq data of antioxidant genes indicated that gallic acid may reduce oxidative stress through the glutathione- and thioredoxin-dependent systems in.