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Article Publish Status: FREE
Abstract Title:

Exerts an Anticancer Effect on Human Osteosarcoma Cells via Suppressing the Wnt/β-Catenin Signaling Pathway.

Abstract Source:

Integr Cancer Ther. 2019 Jan-Dec;18:1534735419890917. PMID: 31855073

Abstract Author(s):

Qi-Hao Zhang, Qin-Xiao Hu, Da Xie, Bo Chang, Hou-Guang Miao, Yun-Guo Wang, De-Zhong Liu, Xue-Dong Li

Article Affiliation:

Qi-Hao Zhang

Abstract:

Current treatment of osteosarcoma is limited in part by side effects and low tolerability, problems generally avoided with traditional Chinese medicine., a traditional Chinese medicine with antitumor effects, offers a potential alternative, but little is known about its molecular mechanisms in osteosarcoma cells.To investigate the effect ofon osteosarcoma cells and its mechanism.Osteosarcoma MG63 and U2-OS cells were treated with, followed by assays for cell proliferation (Cell Counting Kit-8), colony formation, and apoptosis (Alexa Fluor 647-Annexin V/propidium iodide, flow cytometry). Migration and invasion of cells were assessed by wound healing and Transwell invasion assays, and the effect ofon Wnt/β-catenin signal transduction was studied by real-time quantitative polymerase chain reaction, western blot, and dual-luciferase assay.inhibited the proliferation, migration, and invasion, and induced apoptosis of human osteosarcoma MG63 and U2-OS cells. Dual-luciferase assay showed thatsuppressed the transcriptional activity of T-cell factor/lymphocyte enhancer factor in the Wnt/β-catenin signaling pathway. Moreover,blocked Wnt/β-catenin signaling by inhibiting the Wnt co-receptor LRP5 and Wnt-related target genes, such as β-catenin, cyclin D1, C-Myc, MMP-2, and MMP-9. At the same time, when Wnt/β-catenin was inhibited, the expression of E-cadherin was upregulated.Our results suggest thatbroadly suppresses osteosarcoma cell growth by inhibiting Wnt/β-catenin signaling.

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