Genistein treatment of cells inhibits arenavirus infection. - GreenMedInfo Summary
Genistein treatment of cells inhibits arenavirus infection.
Antiviral Res. 2008 Feb;77(2):153-6. Epub 2007 Oct 8. PMID: 17961732
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX 77555, United States. [email protected]
Arenaviridae is a family of enveloped viruses some of which are capable of causing hemorrhagic fever syndromes in humans. In this report, we demonstrate that treatment of host cells with the tyrosine kinase inhibitor genistein inhibits infection of cells with the New World arenavirus Pichindé (PICV). The greatest degree of inhibition was observed in pre-treated target cells, but modest inhibition of infection was also seen when drug was added to cultures up to 48h after infection. We show that PICV-induced phosphorylation of the activating transcription factor-2 protein (ATF-2) and cyclic adenosine monophosphate response element binding protein (CREB) is inhibited following genistein treatment. Lastly, genistein treatment also inhibited transduction of cells with pseudotyped retrovirus particles expressing envelope proteins of the Old World arenavirus Lassa virus. These resultsdemonstrate that kinase activity is required for arenavirus infection and that therapeutics designed to inhibit kinase activity should be explored.