L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage.
Plants (Basel). 2019 Nov 29 ;8(12). Epub 2019 Nov 29. PMID: 31795413
Amélia M Silva
L. leaf extracts and herbal infusions are used worldwide due to the health benefits that are attributed to its use, including anti-neoplastic, anti-aging, neuro-protection, antioxidant and others. The aim of this study was to evaluate the effect of an aqueousextract on HepG2 cell viability, genotoxicity and DNA protection against paraquat-induced oxidative damage. Exposure to paraquat (PQ), over 24 h incubation at 1.0 and 1.5µM, did not significantly reduce cell viability but induced concentration and time-dependent oxidative DNA damage.leaf extract produced dose-dependent cytotoxicity (IC= 540.8± 40.5 µg/mL at 24 h exposure), and short incubations (1 h) produced basal and oxidative DNA damage (>750 and 1500µg/mL, respectively). However, lower concentrations (e.g., 75 µg/mL) ofleaf extract were not cytotoxic and reduced basal DNA damage, indicating a protective effect at incubations up to 4 h. On the other hand, longer incubations (24 h) induced oxidative DNA damage. Co-incubation of HepG2 cells for 4 h, withleaf extract (75µg/mL) and PQ (1.0 or 1.5 µM) significantly reduced PQ-induced oxidative DNA damage. In conclusion, the consumption ofleaf extract for long periods at high doses/concentrations is potentially toxic; however, low doses protect the cells against basal oxidative damage and against environmentally derived toxicants that induce oxidative DNA damage.