Ginsenoside Rb1 alleviates ox-LDL-induced vascular endothelium senescence via the SIRT1/Beclin-1/autophagy axis.
J Cardiovasc Pharmacol. 2019 Oct 25. Epub 2019 Oct 25. PMID: 31658172
Oxidative low-density lipoprotein (ox-LDL) induces endothelium senescence and promotes atherosclerosis. Ginsenoside Rb1 (gRb1) has been proved to protect HUVECs, but its effect on ox-LDL induced endothelium senescence and underlying mechanism remains unknown. This study is to explore the involvement of the SIRT1/Beclin-1/autophagy axis in the effect of gRb1 on protecting endothelium against ox-LDL induced senescence. Hyperlipidemia of Sprague Dawley rats was induced by high fat diet and gRb1 was intraperitoneal injected. Senescence model of HUVECs induced by ox-LDL was also established. The results showed that gRb1 alleviated hyperlipidemia-induced endothelium senescence and ox-LDL-induced HUVECs senescence. GRb1 also restored the reductions in SIRT1 and autophagy, which were involved in the anti-senescence effects. Beclin-1 acetylation was reduced, and the correlation between SIRT1 and Beclin-1 was increased by gRb1. Results of our study demonstrated the anti-senescence function of gRb1 against hyperlipidemia in the endothelium, and the underlying mechanism involves the SIRT1/Beclin-1/autophagy axis.