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Abstract Title:

Studies of the effects and mechanisms of ginsenoside Re and Rkon myelosuppression induced by cyclophosphamide.

Abstract Source:

J Ginseng Res. 2019 Oct ;43(4):618-624. Epub 2018 Aug 30. PMID: 31695568

Abstract Author(s):

Jiahong Han, Jing Xia, Lianxue Zhang, Enbo Cai, Yan Zhao, Xuan Fei, Xiaohuan Jia, He Yang, Shuangli Liu

Article Affiliation:

Jiahong Han

Abstract:

Background: Ginsenoside Re (Re) is one of the major components ofMeyer. Ginsenoside Rk(Rk) is a secondary metabolite of Re. The aim of this study was to investigate and compare the effects and underlying mechanisms of Re and Rkon cyclophosphamide-induced myelosuppression.

Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide. Peripheral blood cells, bone marrow nucleated cells, and colony yield of hematopoietic progenitor cellswere counted. The levels of erythropoietin, thrombopoietin, and granulocyte macrophage colony-stimulating factor in plasma were measured by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. The expression of apoptotic protein bcl-2, bax, and caspase-3 was detected by Western blotting.

Results: Both Re and Rkcould improve peripheral blood cells, bone marrow nucleated cell counts, thymus index, and spleen index. Furthermore, they could enhance the yield of colonies culturedand make the levels of granulocyte macrophage colony-stimulating factor, erythropoietin, and thrombopoietin normal, reduce the ratio of G/Gphase cells, and increase the proliferation index. Finally, Re and Rkcould upregulate the expression of bcl-2, whereas they could downregulate the expression of bax and caspase-3.

Conclusion: Re and Rkcould improve the hematopoietic function of myelosuppressed mice. The effect of Rkwas superior to that of Re at any dose. Regulating the levels of cytokines, promoting cells enter the normal cell cycle, regulating the balance of bcl-2/bax, and inhibiting the expression of caspase-3 may be the effects of Re and Rkon myelosuppression.

Study Type : Animal Study

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