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Abstract Title:

[Effect of Ginsenoside Rg2 and Its Stereoisomers on Oxygen-Glucose Deprivation and Reperfusion Induced Cortical Neuronal Injury Model].

Abstract Source:

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Mar ;36(3):333-8. PMID: 27236892

Abstract Author(s):

Ming-shan Pi, Qin Ru, Xiao-kang Gong, Ri-hui Wu, Xiang Tian, Xiong Qi, Chao-ying Li

Article Affiliation:

Ming-shan Pi

Abstract:

OBJECTIVE: To observe the effect of natural type ginsenoside Rg2 (Rg2) and its stereoisomers [20 (R)-Rg2 and 20 (S)-Rg2] at different concentrations on oxygen-glucose deprivation/ reperfusion (OGD/R) induced cortical neuronal injury model in vitro, and to explore the mechanism, and compare their differences of action.

METHODS: Cortical neurons after 7-day culture were randomly divided into 5 groups, i.e., the control group, the model group, the Rg2 group, 20 (R) -Rg2 group, and 20 (S) - Rg2 group. Cortical neurons in the Rg2 group, 20 (R)-Rg2 group, and 20(S)-Rg2 group were pretreated with 20, 40, and 80μmol/L Rg2, 20 (R) -Rg2, and 20 (S) -Rg2 for 24 h to prepare OGD/R model. The cell survival rate, the activity of Caspase-3, the intracellular Ca2+ concentration, contents of superoxide dismutase (SOD) and malondialdehyde (MDA) were detected 24 h later.

RESULTS: Compared with the control group, cell survival rates and activities of SOD obviously decreased, the activity of Caspase-3, Ca2+ fluorescent optical gray value, and contents of MDA significantly increased with statistical difference (P<0.05). Compared with the model group, cell survival rates and activities of SOD obviously increased, the activity of Caspase-3, Ca2+ fluorescent optical gray value, and contents of MDA significantly decreased in 20μmol/L Rg2 group, 40 μmol/L 20 (R) -Rg2 group, and 80 μmol/L 20 (S) -Rg2 group (P<0.05). Compared with 20(S)-Rg2 group, cell survival rates increased and contents of MDA significantly decreased in 20, 40, and 80μmol/L Rg2 and 20 (R)-Rg2 groups (P<0.05). The activity of Caspase-3 decreased and contents of SOD increased in 80μmol/L 20 (R)-Rg2 group, and 40, 80 μmol/L Rg2 groups (P<0.05). Ca2+ fluorescent optical gray value decreased in 40, 80μmol/L Rg2 and 20 (R)-Rg2 groups (P<0.05). Compared with 20 (R)-Rg2 group, Ca2+ fluorescent optical gray value decreased in 80μmol/L Rg2 group (P<0.05); contents of SOD increased in 40 and 80μmol/L Rg2 groups (P<0.05); contents of MDA decreased in 20, 40, and 80μmol/L Rg2 groups (P<0.05).

CONCLUSIONS: Rg2 and its stereoisomers could improve cell vitality of cortical neurons against OGD/R induced injury. This might be related to improving anti-apoptotic capacities and antioxidant abilities, and reducing Ca2+ inflow. Besides, the neuroprotective effect of 20 (R) -Rg2 was better than that of 20 (S) -Rg2, but inferior to that of Rg2.

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Sayer Ji
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