Effect of ginsenoside Rhon renal apoptosis in cisplatin-induced nephrotoxicity in vivo.
Phytomedicine. 2019 Feb 6 ;61:152862. Epub 2019 Feb 6. PMID: 31048124
BACKGROUND: Ginsenoside Rh(Rh), an important ingredient from Panax ginseng, has received much attention due to a range of pharmacological actions.
PURPOSE: The aim of the study was to investigate the therapeutic potential Rhon cisplatin (CDDP)-induced nephrotoxicity and to elucidate involved mechanisms.
STUDY DESIGN: An in vivo mice model of CDDP-induced nephrotoxicity was established by a single intraperitoneal injection of CDDP (20 mg/kg) to assess the effects of Rhon renal biochemical parameter, oxidative stress, inflammation tubular cell apoptosis and serum metabolic profiles.
RESULTS: Rhprotected against CDDP-induced renal dysfunction and ameliorated CDDP-induced oxidative stress, histopathological damage, inflammation and tubular cell apoptosis in kidney. Rhtreatment had significantly increased expression of Bcl-2 and decreased expression of p53, Bax, cytochrome c, caspase-8, caspase-9, and caspase-3 in kidney tissues. Metabolomic analysis identified 29 altered serum metabolites in Rhtreatment mice.
CONCLUSION: These results suggest that Rhprotects against CDDP-induced nephrotoxicity via action on caspase-mediated pathway.