Protective effect of Glycine max and Chrysanthemum indicum extracts against cisplatin-induced renal epithelial cell death.
Hum Exp Toxicol. 2011 Dec ;30(12):1931-44. Epub 2011 Mar 15. PMID: 21406484
UNLABELLED: Although cisplatin is one of the most efficient chemotherapeutic agents for the treatment of solid tumors, frequently observed nephrotoxicity has limited its use in several patients.
MATERIALS AND METHODS: The protective effect of Glycine max (GM) and Chrysanthemum indicum (CM) extracts on cisplatin-induced apoptosis in human proximal tubular HK-2 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Hoechst 33342, and propidium iodide assays. Reactive oxygen species (ROS) were determined by flow cytometry with 2,7-dichlorofluorescein diacetate (DCFH(2)-DA).
RESULTS: Cisplatin-induced renal cell toxicity through the induction of hydrogen peroxide (H(2)O(2)) and hydroxyl radical (OH(•-)). CM extract protected cisplatin-induced apoptosis by its anti-oxidant activity against H(2)O(2) and OH(•-), while GM extract scavenged only H(2)O(2). Furthermore, GM and CM extracts protect renal cells without significant interfering effect on cisplatin toxicity in lung cancer H460 and melanoma G361 cells.
CONCLUSION: GM and CM extracts exhibited a promising protective effect on cisplatin-induced nephrotoxicity which could benefit the development for nephroprotective approaches.