n/a
Abstract Title:

[High dose vitamin C inhibits proliferation of breast cancer cells through reducing glycolysis and protein synthesis].

Abstract Source:

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2019 May 25 ;48(3):296-302. PMID: 31496162

Abstract Author(s):

Qingmei Wang, Qianzi Xu, Anyi Wei, Shishuo Chen, Chong Zhang, Linghui Zeng

Article Affiliation:

Qingmei Wang

Abstract:

OBJECTIVE: To investigate the effects of high dose vitamin C (VC) on proliferation of breast cancer cells and to explore its mechanisms.

METHODS: Human breast cancer cells Bcap37 and MDA-MB-453 were treated with VC at low dose (0.01 mmol/L), medium dose (0.10 mmol/L) and high dose (2.00 mmol/L). Cell proliferation was determined with CCK-8 assay, protein expression was evaluated by Western blot, and the secretion of lactic acid in tumor cells was detected by colorimetric method. Bcap37 cells were inoculated in nude mice, and tumor baring nude mice were intraperitoneally injected with high VC(4 g/kg, VC group,=5)or normal saline (control group,=5) for 24 d. Tumor weight and body weight were calculated.

RESULTS: experiments demonstrated that high dose VC significantly inhibited cell proliferation in Bcap37 and MDA-MB-453 cells (all<0.01); the expressions of Glut1 and mTOR signaling pathway-related proteins were decreased (all<0.05); and the secretion of lactic acid was also markedly reduced (all<0.05).experiment showed that the tumor weight was decreased in mice treated with high-dose VC as compared with control group (<0.05), but no difference in body weights between two groups was observed.

CONCLUSIONS: High dose VC may inhibit proliferation of breast cancer cells bothandthrough reducing glycolysis and protein synthesis.

Study Type : In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.