https://www.ncbi.nlm.nih.gov/pubmed/22200452 - GreenMedInfo Summary
Visceral botulism at dairy farms in Schleswig Holstein, Germany: prevalence of Clostridium botulinum in feces of cows, in animal feeds, in feces of the farmers, and in house dust.
Anaerobe. 2012 Apr ;18(2):221-3. Epub 2011 Dec 21. PMID: 22200452
Institute of Bacteriology and Mycology, University of Leipzig, Leipzig, Germany. firstname.lastname@example.org
From 41 dairy farms in Schleswig Holstein, Germany, 196 fecal specimens of diseased cows, 77 fecal specimens of farmers and family members from 26 of these farms, 35 animal feed specimens and 7 house dust specimens were investigated for Clostridium botulinum and its antigens, respectively. Four of the humans under study (one child, 8 month, and three adults) showed symptoms of infant/visceral botulism. Specimens were cultivated in reinforced clostridial medium (RCM). C. botulinum antigens were detected by ELISA. The aim of the study was to obtain information on the relationship of detected C. botulinum toxin-types in cows, in the feces of attending humans, and in the immediate environment. The results revealed that C. botulinum toxin-types were different for cows and humans. Toxin-type A was dominant in cow feces while type E was found in humans. Type E was also present in some animal feed specimens. Conversely, toxin-type A was prevalent in the house dust of farms. It may be assumed that the feeds were the source of human colonization with C. botulinum.