Protective Effect ofExtract against Dextran-Sulfate-Sodium-Induced Ulcerative Colitis in Rats.
Nutrients. 2019 Sep 29 ;11(10). Epub 2019 Sep 29. PMID: 31569451
Mohamed A Morsy
Inflammatory bowel disease is a multifactorial inflammatory condition. This study aimed to test the protective effects ofagainst ulcerative colitis (UC). UC was induced in thirty-six male Wistar rats by adding dextran sulfate sodium (DSS) to their drinking water, while a control group received only drinking water. UC rats were equally-divided into six groups that received a single oral daily dose of vehicle (DSS), sulfasalazine (SSZ, 50 mg/kg/day), chloroform or the hydroalcoholic extracts of(100 or 200 mg/kg/day) for 15 days, and then blood and colon samples were harvested for determination of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), erythrocyte sedimentation rate (ESR), myeloperoxidase (MPO), and histopathology. At the end of the study, compared to time-matched controls, UC rats showed increased TNF-α (1.64-fold), IL-6 (5.73-fold), ESR (3.18-fold), and MPO (1.61-fold), along with loss of bodyweight (24.73%) and disease activity index (1.767 ± 0.216 vs. 0 ± 0),<0.001. These effects were prevented by SSZ treatment (<0.001 vs. DSS). The hydroalcoholic extract ofdose-dependently modulated all DSS-induced inflammatory changes. However, the chloroform extract significantly lowered only IL-6 and ESR, but not TNF-α or MPO levels. The protective effects of the hydroalcoholic extract ofagainst experimental UC involved mitigation of DSS-induced inflammation.