Immune modulatory and antioxidant effects of locally administrated vitamin C in experimental periodontitis in rats.
Acta Odontol Scand. 2020 Mar 11:1-8. Epub 2020 Mar 11. PMID: 32157939
Vitamin C is an important water-soluble vitamin with antioxidant and immune-modulatory actions. The aim of this study was to investigate the effects of locally applied vitamin C on alveolar bone resorption in rats with experimental periodontitis.Twenty-one male Sprague-Dawley rats divided into three groups with seven animals in each group: (1) control, (2) experimental periodontitis and 3) experimental periodontitis-local vitamin C treatment group. After ligature was removed, 50 μL vitamin C was locally administered into the subperiosteum of the buccal gingiva of periodontitis vitamin C (PvitC) group rats for three times in intervals of 2 days. At the end of the study, the animals were scarified, and serum and gingival samples were collected for analysis of serum IL-1β, oxidative stress index (OSI), CTX and malondialdehyde (MDA) levels and gingival MMP-8 immunostaining. Alveolar bone loss and attachment loss were determined based on measurements on histological sections obtained from rat mandibles.Serum MDA and OSI levels which are related to the oxidative stress were significantly lower in the PvitC group as compared with those in the P group ( < .05). Serum CTX levels which are related to the bone resorption were significantly lower in the PvitC group as compared with those in the P group ( < .05). The numeric density of MMP-8-positive cells was significantly lower in the PvitC group compared to P group ( < .05). Alveolar bone loss and attachment loss were significantly lower in the PvitC group compared to P group ( < .05)The local vitamin C administration provided protection against inflammation-induced alveolar bone resorption by decreasing oxidative stress and inflammation-induced tissue breakdown vitamin C may be a therapeutic agent that can be used in periodontitis treatment.