Immunomodulatory Effect of Flavonoids of Blueberry (L.) Leaves via the NF-B Signal Pathway in LPS-Stimulated RAW 264.7 Cells.
J Immunol Res. 2017 ;2017:5476903. Epub 2017 Dec 27. PMID: 29445755
Objective: This study aimed to explore the immunoregulatory effect of flavonoids of blueberry (L.) leaves (FBL).
Methods: The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of FBL were investigated in lipopolysaccharide- (LPS-) induced RAW 264.7 cells.
Results: According to the results of UPLC/Q-Tof-MS, nine flavonoids of blueberry leaves were identified. FBL showed a significant reduction in the production of TNF-in LPS-stimulated RAW 264.7 cells. FBL significantly decreased the expression of NF-B p65 and P-NF-B p65 in LPS-induced RAW 264.7 cells in a dose-dependent manner.
Conclusion: Our study showed the immunoregulatory effect of FBL through the suppression of TNF-via the NF-B signal pathway.