The Effect of Various Doses of Oral Vitamin DSupplementation on Gut Microbiota in Healthy Adults: A Randomized, Double-blinded, Dose-response Study.
Anticancer Res. 2020 Jan ;40(1):551-556. PMID: 31892611
BACKGROUND/AIM: To investigate the effects of vitamin Dsupplementation on gut microbiota.
PATIENTS AND METHODS: Twenty adults with vitamin D insufficiency/deficiency [25(OH)D<30 ng/ml] were enrolled and given 600, 4,000 or 10,000 IUs/day of oral vitamin DStool samples were collected at baseline and 8 weeks for identifying gut microbiota using 16S rRNA gene amplification and sequencing.
RESULTS: Baseline serum 25(OH)D was associated with increased relative abundance of Akkermansia and decreased relative abundance of Porphyromonas (p<0.05). After the intervention, we observed a dose-dependent increase in relative abundance of Bacteroides with a significant difference between the 600 IUs and the 10,000 IUs groups (p=0.027), and Parabacteroides with a significant difference between the 600 IUs and the 4,000 IUs groups (p=0.039).
CONCLUSION: Increased serum 25(OH)D was associated with increased beneficial bacteria and decreased pathogenic bacteria. A dose-dependent increase in bacteria associated with decreased inflammatory bowel disease activity was observed after vitamin Dsupplementation.