Effect ofCrude Bark Extracts Concentrations against Gram-Positive and Gram-Negative Bacterial Pathogens.
J Pharm Bioallied Sci. 2019 Jan-Mar;11(1):33-37. PMID: 30906137
Background: Today's pharmaceutical markets are crowded with antibacterial agents, yet their toxicities and resistance are always contentious.is used in India as an age-old treatment for diverse medical conditions. This study was put forth to analyze the antibacterial action ofbark extract on different bacterial pathogens.
Materials and Methods: bark extract was prepared using Soxhlet extraction method. The bark extract ofwas screened for antibacterial activity by agar well diffusion assay technique against,,, and Proteus mirabilis. Ciprofloxacin (5µg per disc) was used as positive control. All assays were carried out under strict aseptic precautions. All the concentrations included were duplicated, and the results obtained were the average of two independent experiments. Broth microdilution method was used to study the minimum inhibitory concentration of the bark extract of.
Results: bark extract has shown antibacterial activity against all concentrations of bark extract, whereas antibacterial activity againstwas observed at higher concentration of>500µg/mL. The bark extract ofshowed zone of clearance against,, andat all concentrations. Zone of inhibition was observed in higher concentrations against.
Conclusion: This study has shown thatbark extract has a potent antibacterial property against,,, and. Further isolation of bioactive compounds may lead to a new scope in the treatment of bacterial infections.