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Abstract Title:

Effect of indole-3-carbinol on transcriptional profiling of wound-healing genes in macrophages of systemic lupus erythematosus patients: an RNA sequencing assay.

Abstract Source:

Lupus. 2020 Jul ;29(8):954-963. Epub 2020 Jun 9. PMID: 32517571

Abstract Author(s):

Farnaz Eghbalpour, Mehrdad Aghaei, Mansour Ebrahimi, Mohammad Reza Tahsili, Masoud Golalipour, Saeed Mohammadi, Yaghoub Yazdani

Article Affiliation:

Farnaz Eghbalpour

Abstract:

BACKGROUND: Relapses and flares with delayed wound healing are among the main symptoms of systemic lupus erythematosus (SLE), a rheumatic autoimmune disease. The orientation of immune responses in SLE disease depends on the function of the population of macrophages. This study investigated the effect of indole-3-carbinol (I3C) on transcriptional profiling of macrophage-derived monocytes (MDMs) in four stages of the wound-healing process.

METHODS: In the first phase of study, MDMs were generated from peripheral blood mononuclear cells of three new SLE cases (unmedicated) and two healthy controls. The cases and controls were then divided into I3C treated and untreated groups after 24 hours of exposure to I3C. Single-end RNA sequencing was performed using an Illumina NextSeq 500 platform. After comprehensive analysis among differentially expressed genes,,andwere validated by quantitative real-time polymerase chain reaction as upregulated ranked genes involved in wound-healing stages.

RESULTS: The RNA sequencing analysis of treated cases and treated controls versus untreated cases and untreated controls (group 3 vs. group 4) revealed upregulation of various genes, for example:,,,,,andin coagulation;,andin M2 reprogramming;,,andin extracellular matrix organization; and,andin the proliferation stage of wound healing.

CONCLUSIONS: The results showed that treatment with I3C could modulate the gene expression involved in wound healing in SLE cases and healthy controls.

Study Type : Human In Vitro, Human Study

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