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Article Publish Status: FREE
Abstract Title:

Induction of Apoptosis and Growth-Inhibition by Thymoquinone in ACHN and GP-293 Cell Lines in Comparable with Cis-Platinum.

Abstract Source:

J Pharmacopuncture. 2019 Sep ;22(3):176-183. Epub 2019 Sep 30. PMID: 31673449

Abstract Author(s):

Samira Shahraki, Reza Mohebbati, Mohammad Naser Shafei, Mahmoud Mahmoudi, Sara Hosseinian, Soghra Parhizgar, Zohreh Naji Ebrahimi Yazd, Nazanin Entezari Heravi, Reza Nejad Shahrokh Abadi, Abolfazl Khajavirad

Article Affiliation:

Samira Shahraki

Abstract:

Objective: In the current work, we investigated the cytotoxic and apoptotic effects of Thymoquinone (TQ), an active compound of Nigella sativa (N. sativa) and Cis-platinum, on normal renal epithelial (GP-293) and human renal adenocarcinoma cell lines (ACHN).

Methods: GP-293 and ACHN cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% Fetal bovine serum (FBS) and 1% penicillin plus streptomycin antibiotic. The MTT assay was used for cellular viability assessment. Viability of cells was observed using inverted light microscope 24, 48 and 72 h after exposure of the cells to various concentrations of TQ (1, 2.5, 5, 10, 50 and 100μg/ml) and Cis-platinum (0.5, 1, 1.5, 2, 3, 6 and 12.5 μg/ml). Moreover, apoptosis was analyzed with a flow-cytometry method. The untreated cells were considered as control group.

Results: Morphological changes such as reduced cell number and increased intercellular distance and reduced cell viability in ACHN and GP-293cell lines were observed in both TQ and Cis-platinum groups; however, Cis-platinum had greater effect on ACHN cell line than GP-293 cell line. In addition, GP-293 cell line was more sensitive to TQ compared to ACHN cell line. Furthermore, TQ and Cis-platinum had apoptotic effects on both ACHN and GP-293 cell lines.

Conclusion: Our findings demonstrated that TQ and Cis-platinum had cytotoxic and apoptotic effects on both cell lines, However, GP-293 cell line was more sensitive to TQ. Additionally, Cis-platinum was more effective on ACHN cell line than on GP-293 cell line.

Study Type : In Vitro Study

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