Inhibitory effect of crocin against gastric carcinoma via regulating TPM4 gene. - GreenMedInfo Summary
Inhibitory Effect of Crocin Against Gastric Carcinoma via Regulating TPM4 Gene.
Onco Targets Ther. 2021 ;14:111-122. Epub 2021 Jan 7. PMID: 33442270
Background: Gastric cancer (GC) is one of the most common malignant tumors and the second most frequent cause of cancer death worldwide. Crocin is a kind of bioactive constituent found in the stigmas of saffron, which has shown various pharmacological activities.
Methods: In this study, we investigated the inhibitory effect of crocin on gastric cancer AGS cells proliferation and explored the underlying mechanism. A series of methods were used including cell counting kit assay, gene microarray analysis, qRT-PCR, Celigo image cytometry, cell clone formation assay, Western blot, and cell xenograft growth in vivo.
Results: The results indicated that crocin inhibited AGS cells proliferation and promoted cell apoptosis. Further studies suggested that crocin decreased a series of genes expression, among whichgene downregulation inhibited the tumor cells proliferation and tumor growth in mice, and overexpression ofgene abolishes the inhibitory effect of crocin. Further study using microarray analysis suggested that knocking down ofaltered genes related to the proliferation and apoptosis of cells.
Discussion: Crocin could inhibit the gastric cancer cells AGS cells proliferation by regulatinggene expression, andmay be a promising therapeutic target for GC treatment.