Isolation and characterization of opuntiol from(L. Mill) and its antiproliferative effect in KB oral carcinoma cells.
Nat Prod Res. 2019 Nov 12:1-5. Epub 2019 Nov 12. PMID: 31711321
Ponniresan Veeramani Kandan
In this study, we isolated and characterized a novel bioactive flavonol from the cactus pad ofIndica (L. Mill) (OFI) by chromatography techniques. The isolated compound was characterized by FT-IR,H andC NMR spectroscopy. Single-crystal XRD results illustrate that the obtained flavonol was opuntiol (6-hydroxymethyl-4-methoxy-2-pyran-2-one) and it was found to be near planar except for the H atoms of the methylene and methyl groups. The crystal packing was stabilized by C-H….O and O-H….O intermolecular hydrogen bonds. The isolated opuntiol significantly inhibited KB cells proliferation and its ICvalue was found to be 30 µM. Further, we noticed that opuntiol significantly induced ROS generation and subsequently altered MMP in KB cells. Western blot analysis and morphological observations by fluorescence microscope indicate the apoptotic inducing potential of opuntiol in KB cells.