Abstract Title:

In-Vitro Anti-Proliferative, Apoptotic And Antioxidative Activities Of Medicinal Herb Kalonji (Nigella sativa).

Abstract Source:

Curr Pharm Biotechnol. 2019 Aug 21. Epub 2019 Aug 21. PMID: 31433749

Abstract Author(s):

Tahir Maqbool, Sana Javaid Awan, Sabeen Malik, Faheem Hadi, Somia Shehzadi, Kanza Tariq

Article Affiliation:

Tahir Maqbool


BACKGROUND: Natural product with apoptotic activity could serve as potential new sources for anti-cancer medicine. Numerous phytochemicals from plants have shown to exert antineoplastic effects via programmed cell death (apoptosis). Cancer is one of the leading causes of death in the prosperous countries. The subject study was intended to evaluate the anticancer properties of Kalonji extracts against cancer cell lines HeLa and HepG2 and normal cell lines BHK and VERO were used as normal controls.

RESULTS: For the evaluation of anti-proliferative effects, cell viability and cell death in all groups of cells was evaluated via MTT, crystal violet and trypan blue assays. For the evaluation of Angiogenesis immunocytochemistry and ELISA of VEGF were done. Immunocytochemistry and ELISA of Annexin-V and p53 were performed for the estimation of apoptosis in all groups of cells. Furthermore LDH assay, Antioxidant enzymes activity (GSH, APOX, CAT and SOD) and Real Time PCR with proliferative and apoptotic markers along with internal control were also performed. Cancer cells of both cell lines HepG2 and HeLa cells showed reduced viability, angiogenesis and proliferation with increased apoptosis when treated with Kalonji extracts. Whereas anti-oxidative enzymes show enhanced levels in treated cancer cells as compared to untreated ones.

CONCLUSION: It was observed that Kalonji extracts have ability to induce apoptosis and improve the antioxidant status of HeLa and HepG2 cells. They can also inhibit the proliferation and angiogenesis in both these cancer cell lines.

Print Options

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.