Prevention of hormonal breast cancer by dietary jamun.
Mol Nutr Food Res. 2016 Mar 31. Epub 2016 Mar 31. PMID: 27030099
SCOPE: Syzygium cumini (Jamun) is perhaps the only berry that has the diversity of anthocyanidins of blueberry and bilberry and the abundance of ellagitannins/ellagic acid of black raspberry. Here we report the potential of jamun against 17β-estrogen-mediated breast cancer and the role of miRNAs and other targets in disease inhibition.
METHODS AND RESULTS: Female ACI rats were given AIN-93M diet or diet supplemented with jamun. Two weeks later, animals received 17β-estradiol and were palpated weekly for the mammary tumors. At the end of 26 week, the jamun-diet significantly delayed the first tumor appearance by 21 days, and reduced the tumor incidence (65% vs 96%), tumor burden (313±95 vs 661±123 mm(3) ) and tumor multiplicity (1.8±0.3 vs 4.2±0.4 tumors/rat) compared to control. The experimental diet significantly reduced the estrogen-associated growth of pituitary prolactinomas, circulating prolactin and estradiol levels and offset estrogen-associated increases in mammary cell-proliferation, ER-α and cyclinD1. MiRNAs that were either overexpressed (miR-182 and miR-375) or underexpressed (miR-127 and miR-206) following estrogen-treatment were significantly protected by jamun diet.
CONCLUSIONS: Together, our data show that jamun significantly offset estrogen-mediated alterations in mammary cell-proliferation, ER-α, cyclinD1, and candidate miRNAs, and that the modulation of these biomarkers correlated with a reduction in mammary carcinogenicity. This article is protected by copyright. All rights reserved.