n/a
Abstract Title:

Evaluation of keratin biomaterial containing silver nanoparticles as a potential wound dressing in full-thickness skin wound model in diabetic mice.

Abstract Source:

J Tissue Eng Regen Med. 2019 Dec 11. Epub 2019 Dec 11. PMID: 31825159

Abstract Author(s):

Marek Konop, Joanna Czuwara, Ewa Kłodzińska, Anna K Laskowska, Dorota Sulejczak, Tatsiana Damps, Urszula Zielenkiewicz, Iwona Brzozowska, Antonio Sureda, Tomasz Kowalkowski, Robert A Schwartz, Lidia Rudnicka

Article Affiliation:

Marek Konop

Abstract:

Keratin is a cytoskeletal scaffolding protein essential for wound healing and tissue recovery. The aim of the study was to evaluate the potential role of insoluble fur keratin-derived powder containing silver nanoparticles (FKDP-AgNP) in the allogenic full-thickness surgical skin wound model in diabetic mice. The scanning electron microscopy image evidenced the keratin surface is covered by a single layer of silver nanoparticles. Data obtained from dynamic light scattering and micellar electrokinetic chromatography showed three fractions of silver nanoparticles with an average diameter of 130, 22.5 and 5 nm. Microbiologic results revealed that the designed insoluble FKDP-AgNP dressing to some extent the growth of Escherichia coli and Staphylococcus aureus. In vitro assays showed that the FKDP-AgNP dressing did not inhibit fibroblast growth or induce hemolysis. In vivo studies using a diabetic mice model confirmed biocompatible properties of the insoluble keratin dressings. FKDP-AgNP significantly accelerated wound closure and epithelization at day 5 and 8 (p < 0.05) when compared with controls. Histological examination of the inflammatory response documented that FKDP-AgNP-treated wounds contained predominantly macrophages while their untreated variants showed mixed cell infiltrates rich in neutrophils. Wound inflammatory response based on macrophagesfavors tissue remodeling and healing. In conclusion, the investigated FKDP-AgNP dressing consisting of an insoluble fraction of keratin which is biocompatible, significantly accelerated wound healing in a diabetic mouse model.

Study Type : Animal Study, In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.