Protective Effect ofLA12 in an Alcohol-Induced Rat Model of Alcoholic Steatohepatitis.
Korean J Food Sci Anim Resour. 2017 ;37(6):931-939. Epub 2017 Dec 31. PMID: 29725216
Alcoholic liver disease (ALD) is a complex multifaceted disease that involves oxidative stress and inflammation as the key mediators. Despite decades of intensive research, there are no FDA-approved therapies, and/or no effective cure is yet available. Probiotics have received increasing attention in the past few years due to their well-documented gastrointestinal health-promoting effects. Interestingly, emerging studies have suggested that certain probiotics may offer benefits beyond the gut.LA12 has been previously demonstrated to play a role in inflammatory-related disease. However, the possible protective effect ofLA12 on ALD still remain to be explored. Thus, the aim of this study was to evaluate the possible protective effect ofLA12 on alcohol-induced gut barrier dysfunction and liver damage in a rat model of alcoholic steatohepatitis (ASH). Daily oral administration ofLA12 in rat model of ASH for four weeks was shown to significantly reduced intestinal nitric oxide production and hyperpermeability. Moreover, small intestinal histological- and qRT-PCR analysis further revealed thatLA12 treatment was capable of up-regulating the mRNA expression levels of tight junction proteins, thereby stimulating the restitution of barrier structure and function. Serum and hepatic analyses also revealed that the restoration of epithelial barrier function may prevent the leakage of endotoxin into the blood, subsequently improve liver function and hepatic steatosis in theLA12-treated rats. Altogether, results in this study suggest thatLA12 may be used as a dietary adjunct for the prevention and treatment of ASH.