Study ofCNCM I-1518 in Healthy andColonized Elderly Gut Microbiota.
Front Nutr. 2019 ;6:184. Epub 2019 Dec 10. PMID: 31921877
Consumption of probiotic bacteria can result in a transient colonization of the human gut and thereby in potential interactions with the commensal microbiota. In this study, we used novel PolyFermS continuous fermentation models to investigate interactions of the candidate probiotic strainCNCM I-1518 () with colonic microbiota from healthy elderly subjects using 16S rRNA gene amplicon sequencing and metatranscriptomics, or with microbiota-colonized with(NCTC 13307 andDSM 1296)-an enteropathogen prevalent in the elderly population. Small changes in microbiota composition were detected upon daily addition of, including increased abundances of closely related generaand, and of the butyrate producer. Microbiota gene expression was also modulated bywith distinct response of thetranscriptome and an increase in carbohydrate utilization. However, no inhibitory effect ofwas observed oncolonization in the intestinal models under the tested conditions. Our data suggest that, in theexperimental conditions tested and independent of the host,has modulatory effects on both the composition and function of elderly gut microbiota without affectinggrowth and toxin production.