Lactobacillus plantarum alleviate aflatoxins (Band M) induced disturbances in the intestinal genes expression and DNA fragmentation in mice.
Toxicon. 2018 Mar 21 ;146:13-23. Epub 2018 Mar 21. PMID: 29574215
This study aimed to assess the disturbances in intestinal genes expression and DNA fragmentation in mice treated orally with aflatoxin B(AFB) or aflatoxin M(AFM) and the protective activity of Lactobacillus plantarum (LP). Male Balb/c mice were divided into 6 groups including the control group, the group treated with 2 mg/kg b.w of LP (2 × 10 cfu/mL), the groups treated with AFBor AFM(100 μg/kg b.w), and the groups treated with AFBor AFMduring, after or before LP. Small intestines were collected for the determination of DNA fragmentation, gene expression and target protein content. The results showed that AFBor AFMincreased DNA fragmentation, down regulated the expressions of caspase-3, caspase-9, CYP3A13, Bax and p53 as well as up-regulated the expression of TNF-α and Bcl-2 and their target proteins. LP succeeded to alleviate the disturbances in DNA fragmentation and the expression of these genes. The improvement was more pronounced in the group co-administered with the toxins plus LP. It could be concluded that AFBand AFMinduced disturbances in intestinal function via the disturbances in DNA fragmentation and genes expression. LP induced a potential protective effect and is considered a promising agent against the genotoxicity induced by these mycotoxins.