Article Publish Status: FREE
Abstract Title:

Development of a Novel Experimental In Vitro Model of Isothiocyanate-induced Apoptosis in Human Malignant Melanoma Cells.

Abstract Source:

Anticancer Res. 2016 12 ;36(12):6303-6309. PMID: 27919950

Abstract Author(s):

Theodora Mantso, Aristeidis P Sfakianos, Aithne Atkinson, Ioannis Anestopoulos, Melina Mitsiogianni, Sotirios Botaitis, Sebachedin Perente, Constantinos Simopoulos, Stavros Vasileiadis, Rodrigo Franco, Aglaia Pappa, Mihalis I Panayiotidis

Article Affiliation:

Theodora Mantso


BACKGROUND: Isothiocyanates are constituents of cruciferous vegetables which have been associated with reduced cancer risk partially through their ability to induce apoptosis in malignant cells including melanoma.

MATERIALS AND METHODS: We have utilized human malignant melanoma (A375), epidermoid carcinoma (A431) and immortalized keratinocyte (HaCaT) cells exposed to various isothiocyanates, under different experimental conditions.

RESULTS: An experimental in vitro model utilizing low isothiocyanate concentrations (0.1-5μM for 48 h with all treatments being refreshed after 24h) was shown to be (i) most efficient in exerting an anti-cancer effect when compared to higher concentrations (5-100 μM for 24 or 48 h added as a single bolus) and (ii) specific to A375 cells while A431 and HaCaT cells remained unaffected. Such effect involved the activation of several caspases including (iii) initiator caspases 8, 9, 4 (indicating the involvement of intrinsic, extrinsic and endoplasmic reticulum-based pathways) and (iv) effector caspases 3, 7 and 6.

CONCLUSION: Utilization of low isothiocyanate concentrations (under the conditions described herein) exerts an anti-cancer effect specific to human malignant melanoma cells thus providing a therapeutic basis for their utilization in management of the disease.

Study Type : In Vitro Study

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