Lung anti-oxidant depletion: a predictive indicator of cellular stress induced by ambient fine particles.
Environ Sci Technol. 2020 Jan 21. Epub 2020 Jan 21. PMID: 31961142
Regulations on ambient particulate matter (PM) are becoming more stringent due to adverse health effects arising from PM exposure. PM-induced oxidant production is a key mechanism behind the observed health effects and is heavily dependent on PM composition. Measurement of the intrinsic oxidative potential (OP) of PM could provide an integrated indicator of PM bioreactivity, and could serve as a better metric of PM hazard exposure than PM mass concentration. The OP of two chemically-contrasted PM2.5 samples was compared through four acellular assays and OP predictive capability was evaluated in different cellular assays on two in vitro lung cell models. PM2.5 collected in Paris at site close to the traffic exhibited a systematically higher OP in all assays compared to PM2.5 enriched in particles from domestic wood burning. Similar results were obtained for oxidative stress, expression of anti-oxidant enzymes and pro-inflammatory chemokine in human bronchial epithelial and endothelial cells. The strongest correlations between OP assays and cellular responses were observed with the antioxidant (ascorbic acid and glutathione) depletion (OPAO) assay. Multivariate regression analysis from OP daily measurements suggested that OPAO was strongly correlated with PAH at traffic site while it was correlated with potassium for the domestic wood burning sample.