Luteolin ameliorates testis injury and blood-testis barrier disruption. - GreenMedInfo Summary
Luteolin Ameliorates Testis Injury and Blood-Testis Barrier Disruption through the Nrf2 Signaling Pathway and by Up-Regulating Cx43.
Mol Nutr Food Res. 2019 Mar 29:e1800843. Epub 2019 Mar 29. PMID: 30924608
SCOPE: Luteolin, a natural flavonoid, displays protective activities to testicular tissue. However, molecular mechanisms are still unclear. In this study, we aimed to identify the protective effects and underlying mechanisms of Luteolin against triptolide (TP)-induced damage of testicular tissue.
METHODS AND RESULTS: Pre-incubation of Sertoli cells (SCs) with Luteolin resulted in a significant reduction of TP-induced apoptotic cells, which occurred concomitantly with the effective inhibition of reactive oxygen species (ROS) accumulation. Luteolin resulted in a significant reduction in testicular damage and spermatogenesis dysfunction in a mouse model of testicular damage. Mechanistic studies revealed that Luteolin significantly triggered Nrf2 translocation, increased antioxidant response element (ARE)-luciferase reporter activity, and induced antioxidant enzyme expression. Nrf2 siRNA reduced Luteolin-induced protection in SCs. Besides inhibiting apoptosis, Luteolin recovered blood-testis barrier (BTB) integrity by up-regulating Connexin43 (Cx43) expression. Moreover, specifically blocked Cx43 activity completely blocked repairmen of Luteolin to BTB values. In accordance with in vitro results, Luteolin suppressed testicular injury and spermatogenesis dysfunction by activation of Nrf2 and Cx43 in a testicular injury model.
CONCLUSION: Luteolin was identified as a novel active ingredient that contributed to the protective activity in testicular damage through activating the Nrf2 signaling pathway and by up-regulating Cx43. This article is protected by copyright. All rights reserved.