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Abstract Title:

[TLR/NF-κB independent signaling pathway in TNF-α suppression of diabetic MyD88-knockout mice afterpolysaccharides administration].

Abstract Source:

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2018 Jan 25 ;47(1):35-40. PMID: 30146809

Abstract Author(s):

Tingting Liu, Lingxiao Wang, Xiaohui Yang, Zhiqing Yao, Huizhen Cai

Article Affiliation:

Tingting Liu

Abstract:

OBJECTIVE: : To investigate the effect ofpolysaccharides (LBPs) on TLR/NF-κB independent pathway and serum tumor necrosis factor (TNF-α) level in diabetic MyD88-knockout mice.

METHODS: : Diabetes was induced by feeding high-fat/high-sugar diet and injection of low-dose streptozotocin in MyD88-knockout mice. The diabetic mice were randomly divided into model group, positive control group and LBPs group. The expressions of TRAM, TRIF, TRAF6, RIP1 and TNF-α mRNA and proteins in mouse peritoneal macrophages were detected by real-time RT-PCR and Western blotting after LBPs treatment for 3 month. Serum TNF-α was determined with ELISA kit.

RESULTS: : Real time RT-PCR showed that compared with model group, the relative expressions ofandmRNA in macrophages of LBPs group were significantly decreased and expression ofwas significantly increased (all<0.05). Expression of TRAM, TRIF, TRAF6, RIP1 and TNF-α proteins as well as serum TNF-α level had no significant difference between LBPs group and model group (all>0.05).

CONCLUSIONS: : LBPs may not inhibit serum TNF-α level through TLR/NF-κB independent pathway.

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