Lycopene alleviates titanium dioxide nanoparticle-induced testicular toxicity. - GreenMedInfo Summary
Lycopene Alleviates Titanium Dioxide Nanoparticle-Induced Testicular Toxicity by Inhibiting Oxidative Stress and Apoptosis in Mice.
Biol Trace Elem Res. 2021 Aug 16. Epub 2021 Aug 16. PMID: 34396458
PURPOSE: The research was carried out to investigate the possible ameliorative effect of lycopene on TiONPs-induced male reproductive toxicity and explore the possible mechanism.
METHODS: Ninety-six healthy male Institute of Cancer Research (ICR) mice were equally divided into eight groups (control group, 50 mg/kg TiONPs group, 5 mg/kg LYC group, 20 mg/kg LYC group, 40 mg/kg LYC group, 50 mg/kg TiONPs + 5 mg/kg LYC group, 50 mg/kg TiONPs + 20 mg/kg LYC group, 50 mg/kg TiONPs + 40 mg/kg LYC group), and the mice were treated by intragastric administration every day for 30 days in this research. Sperm parameters, testicular histopathology, oxidant and antioxidant enzymes, and cell apoptosis-related protein expression in the testicular tissue were analyzed.
RESULTS: The results showed that TiONPs exposure significantly decreased sperm count and motility, and TiONPs also increased sperm malformation in the epididymis; these characteristics were improved when co-administration with LYC. Testicular histopathological lesions like disorder of germ cells arrange, detachment, atrophy, and vacuolization were observed after TiONPs exposure, and these abnormalities were effectively ameliorated by co-administration with LYC. Oxidative stress was induced by TiONPs exposure as evidenced by increased the level of MDA and decreased the activity of SOD as well as the level of anti-O, and these alterations were effectively prevented by co-administration with LYC. LYC also alleviated TiONPs-induced germ cell apoptosis by inhibiting mitochondrial apoptotic pathway, as shown by the upregulation of Bcl-2, the downregulation of Bax, Cleaved Caspase 3, and Cleaved Caspase 9.
CONCLUSION: LYC could ameliorate TiONPs-induced testicular damage via inhibiting oxidative stress and apoptosis, which could be used to alleviate the testicular toxicity associated with TiONPs intake.