Antibacterial and Anti-inflammatory Potential ofStem Extract.
Open Dent J. 2018 ;12:265-274. Epub 2018 Mar 30. PMID: 29760819
Background: Periodontitis, a chronic inflammatory disease, is the leading cause of tooth loss in adults. Evidence for the anti inflammatory activity ofStem Extract (MSE) in periodontal disease is limited.
Objective: The study aimed to investigate the inhibitory effect of MSE on the growth of periodontopathic bacteria and expression of interleukin (IL)-6 and IL-8 inLipopolysaccharide (LPS)-stimulated human Periodontal Ligament (hPDL) fibroblasts.
Methods: The antimicrobial activities of MSE were tested againstandby the disk diffusion, the minimum inhibitory concentration and the minimal bactericidal concentration methods. Cytotoxicity ofLPS and MSE on hPDL fibroblasts was determined by MTS assay. The expression of cytokines (IL-6 and IL-8) mRNA and proteins in hPDL fibroblasts was measured using the reverse transcription-qPCR and enzyme-linked immunosorbent assay, respectively.
Results: MSE exhibited antibacterial activities againstandwith the zones of inhibition of 10.00± 0.33 mm and 17.33 ± 0.58 mm, respectively. MIC and MBC values for MSE againstwere 62.5μg/ml. The MIC and MBC values againstwere 250μg/mL and 500 μg/ml, respectively.LPS was shown to mediate the expression of pro-inflammatory cytokines in hPDL fibroblasts. However, treatment with MSE concentrations of 2.5 and 5.0μg/ml significantly suppressedLPS-induced IL-6 and IL-8 mRNA and protein expression (<0.05).
Conclusion: The present study demonstrates that MSE has antibacterial activity against two putative periodontal pathogens. MSE suppressed IL-6 and IL-8 expression inLPS-stimulated hPDL fibroblasts, indicating a possible anti-inflammatory effect. Thus, it is a potential adjunctive agent for the treatment of periodontitis.