Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

n/a
Article Publish Status: FREE
Abstract Title:

Antibacterial and Anti-inflammatory Potential ofStem Extract.

Abstract Source:

Open Dent J. 2018 ;12:265-274. Epub 2018 Mar 30. PMID: 29760819

Abstract Author(s):

Ichaya Yiemwattana, Niratcha Chaisomboon, Kusuma Jamdee

Article Affiliation:

Ichaya Yiemwattana

Abstract:

Background: Periodontitis, a chronic inflammatory disease, is the leading cause of tooth loss in adults. Evidence for the anti inflammatory activity ofStem Extract (MSE) in periodontal disease is limited.

Objective: The study aimed to investigate the inhibitory effect of MSE on the growth of periodontopathic bacteria and expression of interleukin (IL)-6 and IL-8 inLipopolysaccharide (LPS)-stimulated human Periodontal Ligament (hPDL) fibroblasts.

Methods: The antimicrobial activities of MSE were tested againstandby the disk diffusion, the minimum inhibitory concentration and the minimal bactericidal concentration methods. Cytotoxicity ofLPS and MSE on hPDL fibroblasts was determined by MTS assay. The expression of cytokines (IL-6 and IL-8) mRNA and proteins in hPDL fibroblasts was measured using the reverse transcription-qPCR and enzyme-linked immunosorbent assay, respectively.

Results: MSE exhibited antibacterial activities againstandwith the zones of inhibition of 10.00± 0.33 mm and 17.33 ± 0.58 mm, respectively. MIC and MBC values for MSE againstwere 62.5μg/ml. The MIC and MBC values againstwere 250μg/mL and 500 μg/ml, respectively.LPS was shown to mediate the expression of pro-inflammatory cytokines in hPDL fibroblasts. However, treatment with MSE concentrations of 2.5 and 5.0μg/ml significantly suppressedLPS-induced IL-6 and IL-8 mRNA and protein expression (<0.05).

Conclusion: The present study demonstrates that MSE has antibacterial activity against two putative periodontal pathogens. MSE suppressed IL-6 and IL-8 expression inLPS-stimulated hPDL fibroblasts, indicating a possible anti-inflammatory effect. Thus, it is a potential adjunctive agent for the treatment of periodontitis.

Print Options


Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2019 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.