Abstract Title:

[Effect of Magnolol on Proliferation and Apoptosis of HL-60 Cells and Its Molecular Mechanism].

Abstract Source:

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Apr ;24(2):388-93. PMID: 27150997

Abstract Author(s):

Ke Fang, Xiao-Fen Yuan, Qiong Liao, Zhi-Yong Zhang, Guan-Hua Song, Qiang Guo, Xia Ren, Guo-Sheng Jiang

Article Affiliation:

Ke Fang

Abstract:

OBJECTIVE: To investigate the effect of magnolol on proliferation and apoptosis of HL-60 cells and its mechanism.

METHODS: MTT assay was used to measure the proliferation of HL-60 cells after treatment with different concentration of magnolol (5, 10, 20, 40, 80 and 160µg/ml). The morphological changes of HL-60 cells were examined by light microscopy, and DAPI staining was performed to observe the nuclear morphology of HL-60 cells. The early cell apoptosis was detected by flow cytometry with Annexin V-FITC/PI double-staining. RT-PCR was carried out to examine themRNA expression of BAX and BCL-2. Western blot was performed to detect the protein expression of caspase family.

RESULTS: The magnolol inhibited HL-60 cell proliferation, and the inhibitory rate of cell proliferation increased significantly in a dose- and time- dependent manner (P<0.05). HL-60 cells became small, even apoptotic bodies appeared after treatment with magnolol. In addition, nuclear condensation or fragmentation could be observed, which is the typical morphological features of apoptosis. When HL-60 cells were treated with 40µg/ml of magnolol for 24 h, the ratio of early apoptotic cells reached to (11.7 ± 2.4) %, which was significant different from control (1.4 ± 1.1) % (P<0.05). RT-PCR results showed that treatment of HL-60 cells with magnolol up-regulated the expression of BAX, whereas down-regulated the expression of BCL-2. Western blot results showed that the cleavages of caspase-3, -8 and -9 were significantly enhanced by magnolol.

CONCLUSION: The magnolol can significantly inhibit the proliferation of HL-60 cells and induce the apoptosis of HL-60 cells, which may occur through up-regulation of BAX, down-regulation of BCL-2 and the activation of caspases.

Study Type : In Vitro Study

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Sayer Ji
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