Malaysian Tualang honey inhibits hydrogen peroxide-Induced endothelial hyperpermeability. - GreenMedInfo Summary
Malaysian Tualang Honey Inhibits Hydrogen Peroxide-Induced Endothelial Hyperpermeability.
Oxid Med Cell Longev. 2019 ;2019:1202676. Epub 2019 Aug 18. PMID: 31531177
Malaysian Tualang honey (TH) is a known therapeutic honey extracted from the honeycombs of the Tualang tree (Koompassia excelsa) and has been reported for its antioxidant, anti-inflammatory, antiproliferative, and wound healing properties. However, the possible vascular protective effect of TH against oxidative stress remains unclear. In this study, the effects of TH on hydrogen peroxide- (HO-) elicited vascular hyperpermeability in human umbilical vein endothelial cells (HUVECs) and Balb/c mice were evaluated. Our data showed that TH concentrations ranging from 0.01% to 1.00% showed no cytotoxic effect to HUVECs. Induction with 0.5 mM HOwas found to increase HUVEC permeability, but the effect was significantly reversed attenuated by TH (<0.05), of which the permeability with the highest inhibition peaked at 0.1%. In Balb/c mice, TH (0.5 g/kg-1.5 g/kg) significantly (<0.05) reduced HO(0.3%)-induced albumin-bound Evans blue leak, in a dose-dependent manner. Immunofluorescence staining confirmed that TH reduced actin stress fiber formation while increasing cortical actin formation and colocalization of caveolin-1 and-catenin in HUVECs. Signaling studies showed that HUVECs pretreated with TH significantly (<0.05) decreased intracellular calcium release, while sustaining the level of cAMP when challenged with HO. These results suggested that TH could inhibit HO-induced vascular hyperpermeability in vitro and in vivo by suppression of adherence junction protein redistribution via calcium and cAMP, which could have a therapeutic potential for diseases related to the increase of both oxidant and vascular permeability.