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Abstract Title:

Mechanistic study on inhibition of porcine pancreaticα-amylase using the flavonoids from dandelion.

Abstract Source:

Food Chem. 2020 Nov 12:128610. Epub 2020 Nov 12. PMID: 33221105

Abstract Author(s):

Yanmei Huang, Peng Wu, Jian Ying, Zhizhong Dong, Xiao Dong Chen

Article Affiliation:

Yanmei Huang

Abstract:

This study was designed to investigate quantitatively the inhibition and molecular mechanism of pancreaticα-amylase exhibited by flavonoids from dandelion to reveal its potential use in relieving postprandial hyperglycemia. The results show that the flavonoids reversibly inhibited the α-amylase in a non-competitive manner with Michaelis-Menten constant (K) and half-inhibitory concentration (IC) value of 10.51 and 0.0067 mg/mL, respectively. The flavonoids present a strong ability to quench the intrinsic fluorescence of α-amylase through static quenching by forming a complex. The values of the binding site (n) at different temperatures were found to be approximately the unity, indicating the presence of a singleclass of molecular binding of the dandelion flavonoids on α-amylase. The positive values of enthalpy and entropy change reveal that the binding was predominately driven by hydrophobic interactions. This study suggests a benefit of incorporating the dandelion flavonoids in making functional foods inmanaging the diet of the diabetes.

Study Type : In Vitro Study

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Sayer Ji
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