Mesenchymal stem cells-derived exosomes ameliorate blue light stimulation in retinal pigment epithelium cells and retinal laser injury by VEGF-dependent mechanism.
Int J Ophthalmol. 2018 ;11(4):559-566. Epub 2018 Apr 18. PMID: 29675371
AIM: To observe the effect of exosomes derived from human umbilical cord blood mesenchymal stem cells (hUCMSCs) on the expression of vascular endothelial growth factor-A (VEGF-A) in blue light injured human retinal pigment epithelial (RPE) cells and laser-induced choroidal neovascularization (CNV) in rats.
METHODS: Exosomes were isolated from hUCMSCs and characterized by transmission electron microscope and Western blot. MSCs-derived exosomes were cultured with RPE cells exposed to blue light. The mRNA and protein expression of VEGF-A were determined by real time-polymerase chain reaction (PCR) and Western blot, respectively. Immunofluorescence assay was used for the detection of the expression level of VEGF-A. We injected different doses of MSCs-derived exosomes intravitreally to observe and compare their effects in a mouse model of laser-induced retinal injury. The histological structure of CNV in rats was inspected by hematoxylin-eosin (HE) staining and fundus fluorescein angiography. The expression of VEGF-A was detected by immunohistochemistry.
RESULTS: Exosomes exhibited the typical characteristic morphology (cup-shaped) and size (diameter between 50 and 150 nm). The exosomes marker, CD63, and hUCMSCs marker, CD90, showed a robust presence., MSCs-derived exosomes downregulated the mRNA(Exo-L:=6.485, 7.959, 9.286; Exo-M:=7.517, 10.170, 13.413; Exo-H:=10.317, 12.234, 14.592,<0.05) and protein (Exo-L:=2.945, 4.477, 6.657; Exo-M:=4.713, 6.421, 8.836; Exo-H:=6.539, 12.194, 12.783;<0.05) expression of VEGF-A in RPE cells after blue light stimulation., we found that the MSCs-derived exosomes reduced damage, distinctly downregulated VEGF-A (Exo-H:=0.957, 1.382;<0.05), and gradually improved the histological structures of CNV for a better visual function (Exo-L: 0.346, Exo-M: 3.382, Exo-H: 8.571;<0.05).
CONCLUSION: MSCs-derived exosomes ameliorate blue light stimulation in RPE cells and laser-induced retinal injurydownregulation of VEGF-A.