Mitochondrial dysfunction in endothelial cells induced by airborne fine particulate matter. - GreenMedInfo Summary
Mitochondrial dysfunction in endothelial cells induced by airborne fine particulate matter (<2.5 μm).
J Appl Toxicol. 2019 Jul 4. Epub 2019 Jul 4. PMID: 31273799
Exposure to ambient fine particulate matter (<2.5 μm; PM) increases the risk of the physiopathology of vascular diseases. However, the underlying mechanism, particularly the mitochondrial damage mechanism, of PM-induced vascular dysfunction is still unclear. In this study, we examined PM-induced alterations of mitochondrial morphology, and further demonstrated the adverse effects on mitochondrial dynamics and function in vascular endothelial cells. Consequently, cultured EA.hy926 cells were subjected to PMcollected from Beijing. A Cell Counting Assay Kit-8 demonstrated that PMexposure decreased the proliferation of EA.hy926 cells in a dose-dependent manner. The exposure caused an increment of abnormal mitochondria coupled with the decrease of fusion protein MFN2 and the increase of fission protein FIS1, suggesting that PMinhibits mitochondrial fusion. Further analyses revealed PMdecreased the mitochondrial membrane potential (ΔΨm) and increased the mitochondrial permeability transport pore opening, eventually resulting in impairments in adenosine triphosphate synthesis. Therefore, it is clearly shown that PMtriggered endothelial toxicity through mitochondria as the target, including the damage of mitochondrial homeostasis.